M. Bolte et al., HARD-TISSUE OF TEETH AND THEIR CALCIUM AND PHOSPHATE CONTENT IN AMBYSTOMA-MEXICANUM (URODELA, AMBYSTOMATIDAE), Annals of anatomy, 178(1), 1996, pp. 71-80
The wall of the pulp cavity, fracture faces and the demineralized surf
aces of teeth from larvae and adults of Ambystoma mexicanum were inves
tigated by scanning electron microscopy (SEM). Calcium and phosphate c
ontents were determined by microanalysis. The apical part of the tooth
(crown, tooth apex) contains dentin canals. In the larva, these do no
t reach the enamel-dentin border but end below this border in front of
a denser hard substance, possibly enameloid. The pedicel in the adult
and the basal portion of the tooth in the larva (base) are without de
ntin canals. These parts of the teeth are characterized by longitudina
lly arranged collagen fibres as visualized on the demineralized surfac
es. These observations indicate a congruency in early-larval and adult
teeth between base and pedicel as well as apex and crown. This partit
ion is also confirmed by the calcium and phosphate values which were i
dentical in larvae and adults. Highest values are found in enamel and
lowest values in the tooth-bearing bone. Calcium and phosphate content
show a clear difference between dentin and the basal part of the toot
h (pedicel and base). The ring-like dividing zone in the adult tooth i
s less well mineralized.