Ma. Valverde et al., DIFFERENTIAL-EFFECTS OF TAMOXIFEN AND I- ON 3 DISTINGUISHABLE CHLORIDE CURRENTS ACTIVATED IN T84 INTESTINAL-CELLS, Pflugers Archiv, 425(5-6), 1993, pp. 552-554
The whole-cell mode of the patch-clamp technique has been used to moni
tor ionic currents in T84 colonic carcinoma cells. The cells were stim
ulated by either a cAMP cocktail, ionomycin or hypotonicity. Sizeable
currents with distinct kinetics were observed after the stimulation wi
th the different agonists. These kinetically distinct Cl- currents als
o presented a differential sensitivity to the anti-oestrogen Tamoxifen
and to the halide I-. Tamoxifen only inhibits the volume activated CI
- current without affecting the other two. Substitution of extracellul
ar CI- by I- shifted the reversal potential towards more negative valu
es both in the hypotonicity and ionomycin activated CI- currents. The
cAMP activated current responded to the CI substitution by T with a bl
ockade of both outward and inward currents, in addition to the displac
ement of the zero current level towards positive values. Thus, the use
of these two simple tools, I- and tamoxifen, allows the distinction o
f CI- channels in epithelial cells.