The inheritance of resistance to barley stripe mosaic virus (BSMV) in
barley was investigated in the Steptoe/Morex doubled haploid populatio
n developed by the North American Barley Genome Mapping Project. The n
umber of resistant (66) and susceptible (84) progeny approximated a 1:
1 ratio, indicating that a single gene was involved in conferring resi
stance to BSMV strain CV42 in Morex barley. This resistance gene was m
apped to the centromeric region of the plus (short) arm of chromosome
1, based on its linkage to molecular markers on the Steptoe/Morex map,
and was found to cosegregate with restriction fragment length polymor
phism (RFLP) marker ABC455. Marker ABG011 was located 6.1 centimorgans
(cM) distal to the resistance locus. The nearest opposite flanking ma
rker mapped was Amy2, located at a distance of 8.1 cM and on the other
side of the centromere. The identification of a cosegregating marker
may facilitate both the selection for BSMV resistance in barley breedi
ng programs and the high resolution mapping of the centromeric region
in the vicinity of this locus.