IMPROVED INTERNALLY STANDARDIZED GC-ECD METHOD FOR THE DETERMINATION OF FREE AND CONJUGATED TRICLOSAN (IRGASAN DP300) IN HUMAN PLASMA

An analytical procedure was developed to determine triclosan and its c onjugates in human plasma. After enzymatic cleavage of the glucuronide and sulfate conjugates, the analyte was separated from matrix constit uents by liquid-liquid extraction. The phenolic hydroxy group of tricl osan was derivatized with acetic anhydride. The resulting ester was ch romatographed on a 60 m GCLC-column. An ECD was used for detection and quantitation. The limit of quantitation for triclosan in plasma was a bout 1 ng/ml. The analytical method was set up to study the pharmacoki netics of triclosan in healthy volunteers following single and repeate d administration of different doses of the compound.