CHARACTERIZATION OF ANTINEUTROPHIL ANTIBODIES IN PATIENTS WITH NEUTROPENIA ASSOCIATED WITH NUTRITIONAL COPPER DEFICIENCY

Six antineutrophil antibody (ANA)-positive patients with copper defici ency were classified into two groups; those with (group A, n = 3) and those without (group B, n = 3) neutropenia. The percent binding of ANA for normal peripheral neutrophils was similar in both groups(83.5 +/- 7.2 vs. 79.1 +/- 10.5%). The percent binding of sera to cultured prom yelocytic leukemia cells (HL-60) was increased in group A (from 3.7 +/ - 3.2 to 12.2 +/- 2.3%) but not in group B (from 65.3 +/- 21.7 to 40.7 +/- 6.3%) after stimulation of HL-60 with DMSO. The stimulated HL-60 cells expressed CD 16 and CD 11b antigens. In the presence of monoclon al antibody for CD 16, the titer of ANA was nil in group A and unchang ed in group B. Thus, ANA of patients with neutropenia may recognize ma inly the CD 16 antigen, the Fc gamma receptor III of neutrophils.