SUB-MILLIMORGAN MAP OF THE PROXIMAL PART OF MOUSE CHROMOSOME-17 INCLUDING THE HYBRID STERILITY-1 GENE

We have generated a high-resolution genetic map, 0.071 cM per backcros s animal, of the 13 cM T-H2 region of the mouse Chromosome (Chr) 17. T he map contains two phenotypic loci, T and Hst1, 12 RFLP markers, and 24 microsatellite loci. The Hstl gene was mapped to a chromosomal inte rval contained within a single 580-kb YAC clone. The FFEH11 YAC is 0.4 4 cM long and carries, besides the Hstl gene, five polymorphic DNA mar kers and recombination breakpoints of six backcross animals. Two candi date genes for Hstl were identified based on their location and testic ular expression. These are Tbp and D17Ph4e. The submilliMorgan map of the T-H2 region revealed significant clustering of (CA)(n) loci. The c lustering, if shown to be a common feature in the mouse genome, may ca use gaps in the physical map of the mouse genome.