Gl. Rorrer et al., DEVELOPMENT AND BIOREACTOR CULTIVATION OF A NOVEL SEMIDIFFERENTIATED TISSUE SUSPENSION DERIVED FROM THE MARINE PLANT ACROSIPHONIA-COALITA, Biotechnology and bioengineering, 49(5), 1996, pp. 559-567
A semidifferentiated tissue culture consisting of linear filaments in
liquid suspension was established from Acrosiphonia coalita, a cold-wa
ter green macroalga known to express pharmacologically active oxylipin
s deriving from lipoxygenase matabolism of linolenic acid. The tissue
was vegatively propagated by blending the filaments down to 1 to 5 mm
in length prior to subculture. The filamentous A, coalita tissue suspe
nsion was successfully cultivated in an illuminated, 3-L stirred-tank
bioreactor at 12 degrees C, 0.46-vvm aeration rate, 250-rpm mixing spe
ed, and incident illumination intensity of 77 mu E m(-2) S-1. The mean
specific growth rate over the exponential phase was 0.185 day(-1) and
a final cell density of 1083 mg dry cell weight (DCW) L(-1) was achie
ved within 15 days of cultivation from an initial cell density of 200
mg DCW L(-1). The addition of 3500 ppm CO2 to the aeration gas provide
d a maximum CO2 transfer rate of six times the maximum CO2 consumption
rate and stabilized the pH to 8.0 during the light phase of growth, b
ut did not improve biomass productivity. (C) 1996 John Wiley & Sons, I
nc.