COMBINED ENZYMATIC-SYNTHESIS OF NUCLEOTIDE (DEOXY) SUGARS FROM SUCROSE AND NUCLEOSIDE MONOPHOSPHATES

The synthesis of NDP-glucose 3a-d (N = A, C, U, dU) with sucrose synth ase B was combined with the enzymatic synthesis of nucleoside diphosph ates 2a-d from their corresponding nucleoside monophosphates 1a-d by d ifferent kinases A. Further combination with recombinant dTDP-glucose 4,6-dehydratase D enabled us to synthesize dUDP-6-deoxy-alpha-D-xylo-4 -hexulose 5 from 1d on a preparative scale. By using the repetitive ba tch technique the enzymatic syntheses of nucleotide (deoxy) sugars 3a- d, 5 could be realized on a 0.1 - 0.5 g scale.