MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY OF FUNGAL METABOLITES - RESOLUTION OPTIMIZED BY EXPERIMENTAL-DESIGN

Fungal metabolites were extracted from two isolates of Penicillium com mune and two isolates of Aspergillus versicolor grown on yeast extract agar (YES) and Czapek yeast extract agar (CYA). Optimized conditions for analysing the extracts by high-performance capillary electrophores is (HPCE) were obtained by experimental designs. The following nine fa ctors were examined by a two-level fractional factorial design: concen tration of the buffer ions PO43- B4O72-, ionic strength of buffer, buf fer pH, addition of sodium dodecyl sulphate (SDS) and sodium deoxychol ate (SCD), addition of acetonitrile and methanol and voltage. Four fac tors significantly influenced the separation of peaks in all the funga l extracts. For optimization of the HPCE method a subsequent response- surface experiment with the important factors was made with an extract from an isolate of Aspergillus versicolor. Optimum separation conditi ons were obtained, which gave good resolution of the components in the extract.