Objective. To determine whether bilateral arthroscopic anterior crucia
te ligament (ACL) transection creates symmetrical osteoarthritis (OA)
in canine knees.Methods. Six dogs underwent bilateral arthroscopic ACL
transections. The animals were sacrificed at intervals ranging from 2
to 12 months post ACL transection. Both knees in each animal were ass
essed for gross pathology, histology, and biochemistry. Results. The l
imited invasiveness of arthroscopic ACL transection allowed bilateral
ACL transections to be performed with minimal animal morbidity. Gross
pathological, histological, and biochemical assessments of bilaterally
ACL transected canine knees consistently confirmed the induction of O
A changes in both knees. Of note, there was no significant difference
in the degree of articular cartilage degeneration created in each pair
of knees. Conclusion. Bilateral ACL transection induces symmetrical c
anine knee OA. This approach provides a potent model for investigating
fundamental OA mechanisms and therapeutic approaches, since one knee
can be experimentally manipulated while the other knee is used as a co
ntrol. This allows each animal to be its own internal control, avoidin
g the interanimal variability associated with the unilateral canine OA
model.