ARSENIC SPECIATION IN SERUM OF UREMIC PATIENTS BASED ON LIQUID-CHROMATOGRAPHY WITH HYDRIDE GENERATION ATOMIC-ABSORPTION SPECTROMETRY AND ONLINE UV PHOTOOXIDATION DIGESTION
X. Zhang et al., ARSENIC SPECIATION IN SERUM OF UREMIC PATIENTS BASED ON LIQUID-CHROMATOGRAPHY WITH HYDRIDE GENERATION ATOMIC-ABSORPTION SPECTROMETRY AND ONLINE UV PHOTOOXIDATION DIGESTION, Analytica chimica acta, 319(1-2), 1996, pp. 177-185
An on-line method was developed for the speciation of arsenic species
in human serum, including monomethylarsonic acid (MMA), dimethylarsini
c acid (DMA), arsenobetaine (AsB) and arsenocholine (AsC). The method
is based on cation-exchange liquid chromatography (LC) separation, UV-
photo-oxidation for sample digestion and continuous hydride generation
(HG) atomic absorption spectrometry (AAS) for the measurement of arse
nic in the LC eluent. By developing the technique of argon segmented f
low in the post-column eluent, an substantial improvement in chromatog
raphic resolution for the separation of these four arsenic species was
obtained. The LC separation, photo-oxidation, hydride generation and
AAS measurement could be completed on-line within 10 min. The detectio
n limits were 1.0, 1.3, 1.5 and 1.4 mu g/l of arsenic for MMA, DMA, As
B and AsC, in serum respectively. The concentration of the 4 species w
as determined in serum samples of patients with chronic renal insuffic
iency. Only AsB and DMA were significantly detected by the present met
hod. The main part of arsenic in human serum is AsB. No MMA, AsC and i
norganic arsenic were detected in these 6 samples.