LIQUID-CHROMATOGRAPHIC DETERMINATION OF TAXOL AND RELATED DERIVATIVESUSING A NEW POLYFLUORINATED REVERSED-PHASE COLUMN

Taxol (paclitaxel) and 5 of its commonly known derivatives (cephaloman nine, 7-epi-10-deacetyltaxol, 10-deacetyltaxol, 10-deacetylbaccatin II I, baccatin III) were separated by a liquid chromatographic method usi ng a new commercially available polyfluorinated reversed-phase column, known as Fluofix(R). The assay achieved good resolution with the exce ption of cephalomannine and 7-epi-10-deacetyltaxol. An injectable form ulation of taxol was validated using this method. The results were con sistent and the procedure could be used for quality control or purity determinations of taxol in bulk and pharmaceutical dosage forms.