DETECTION OF POLYCHLORINATED-BIPHENYLS BY PHOTOACTIVATED FLUORESCENCEAND BY SENSITIZED LASER PHOTOLYSIS

Authors
Citation
Al. Nguyen, DETECTION OF POLYCHLORINATED-BIPHENYLS BY PHOTOACTIVATED FLUORESCENCEAND BY SENSITIZED LASER PHOTOLYSIS, Analytica chimica acta, 319(1-2), 1996, pp. 221-229
Citations number
8
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032670
Volume
319
Issue
1-2
Year of publication
1996
Pages
221 - 229
Database
ISI
SICI code
0003-2670(1996)319:1-2<221:DOPBPF>2.0.ZU;2-E
Abstract
Fluorescence resulting from the photoactivated reaction between polych lorinated biphenyls (PCBs) and diphenylamine in isopropanol solution w as exploited for PCB detection. Photoactivation was performed in 10 mi n with 252 nm light from a xenon are lamp; the same light source, filt ered at 365 nm, provided the excitation energy during fluorescence mon itoring. Small-volume cuvettes were fabricated to enable analysis of 5 mu l samples, and a glass fiber bundle was used to transmit the total emission for detection with a photomultiplier tube to attain a detect ion limit of 0.1 mu g/ml. Sensitization of the PCB photolysis was also investigated by introducing phenothiazine to the reacting solution. A helium-cadmium laser system accomplished the sensitized photolysis in 5 min and the resulting fluorescence was monitored with a fluorometer to allow analysis of 50 mu l samples at 0.05 mu g/ml Both techniques can differentiate between PCBs and non-PCB oil and have been demonstra ted to be applicable for analyzing extracts from spiked soil samples.