THE BIOLOGICAL MONITORING OF MERCURY IN THE SEYCHELLES STUDY

The concentration of total mercury in maternal hair during pregnancy w as used as a measure of fetal exposure to methylmercury in a study of a fish-eating population in the Seychelles islands. A segment of scalp hair approximately 10 centimeters in length, that grew during pregnan cy, was selected for measurement. Total and inorganic mercury were mea sured by cold vapor atomic absorption (CVAA) using the Magos reagents (Magos, 1971). For comparative purposes, total mercury was measured by X-ray Fluorescent Spectrometry (XRF) and methylmercury by gas chromat ography/atomic fluorescence detection (GC/AFD) in a subset of hair sam ples. A limited number of fish samples were also analyzed. Extensive i nterlaboratory testing was conducted to ensure accuracy of the mercury measurements. Concentrations of organic mercury calculated as the dif ference between total and inorganic mercury as measured by CVAA agreed with those of methylmercury measured on the same samples of hair by G C/AFD. Methylmercury measured by GC/AFD and organic mercury measured b y CVAA accounted for over 80% of the total mercury in hair and over 90 % of the total mercury in fish muscle. To test the accuracy of recapit ulation by hair sampling, hair samples were collected from mothers at the time of delivery and 6 months later. The segment corresponding to the pregnancy term was selected for measurement assuming a hair growth rate of 1.1 cm/month. Results from both samples were in close agreeme nt. As part of both a pilot investigation followed by the main study, maternal hair samples were collected each year from 1986 to 1989 for a total of 1604 samples. The median and mean values for each year's col lection fell in the range of 5.9 to 8.2 ppm and exhibited no statistic ally significant trend with time. The highest recorded concentration w as 36 ppm. In hair samples from 654 mothers, the mean concentration of total mercury was compared with the mean concentrations in segments c orresponding to each trimester, approximately 3.3 centimeters in lengt h. A high degree of correlation was found between mean levels in each trimester versus the entire pregnancy segment. (C) 1995 Intox Press, I nc.