A NEW MONOCLONAL-ANTIBODY DETECTING OCHRATOXIN-A AT THE PICOGRAM LEVEL

Authors
GYONGYOSIHORVATH A BARNAVETRO I SOLTI L
Citation
A. Gyongyosihorvath et al., A NEW MONOCLONAL-ANTIBODY DETECTING OCHRATOXIN-A AT THE PICOGRAM LEVEL, Letters in applied microbiology, 22(2), 1996, pp. 103-105
Citations number
14
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
22
Issue
2
Year of publication
1996
Pages
103 - 105
Database
ISI
SICI code
0266-8254(1996)22:2<103:ANMDOA>2.0.ZU;2-N
Abstract
A monoclonal antibody against ochratoxin A was produced after immuniza tion of Balb/c mice with ochratoxin A-BSA. This antibody was of the Ig G1 heavy chain subclass with a kappa type light chain. The 50% inhibit ion value was 0.45 ng ml(-1) in a direct competitive ELISA and the det ection limit was 42 pg ml(-1). This antibody is very specific, cross-r eacting only with ochratoxin B (9.3%).