CHARACTERIZATION OF BETA-MANNANASE AND BETA-MANNOSIDASE SECRETED FROMTHE YEAST TRICHOSPORON-CUTANEUM JCM-2947

beta-Mannanase and beta-mannosidase were purified from the culture flu id of the yeast Trichosporon cutaneum JCM 2947 (=T. beigelii CBS 5790) . The molecular weights of the two enzymes were estimated to be 49 900 and 114 000 by SDS-PAGE and 4500 and 193 000 by gel filtration, respe ctively. beta-Mannanase contained 43% molecular weight as carbohydrate . The K-m and V-max values of beta-mannanase for konjac glucomannan we re 2.7 (mg ml(-1)) and 10.6 (U mg protein(-1)), and those of beta-mann osidase for p-nitrophenyl beta-D-mannopyranoside were 0.25 (mmol l(-1) ) and 91.7 (U mg protein(-1)). Maximal activities were observed betwee n pH 4.0 and 6.5 at 50 degrees C for beta-mannanase and around 6.5 at 40 degrees C for beta-mannosidase.