A method has been developed to determine the free glycerol content in
esters derived from vegetable oils, After extraction of glycerol, its
measurement in small quantities in biofuels (methyl or ethyl eaters) i
s possible by high-pressure liquid chromatography combined with pulsed
amperometric detection, HPLC-PAD, The method has proved to be simple,
rapid and accurate, The limit of detection is 1 mu g/g.