Phylogenetic relationships in Peniophora were investigated using ITS s
equences from nuclear rDNA. The result was compared with parsimony ana
lysis based on morphological characters, and a traditional classificat
ion based on basidiome morphology. Sixty-four specimens belonging to 2
4 species in Peniophora were sequenced. In particular, ITS2 sequences
gave a good resolution in phylogenetic analyses and 3 distinct clades
were distinguished. Intraspecific variation was constantly low in ITSZ
, and rarely exceeded 2%. In ITS1, the variation in Certain species wa
s considerable and hence most confidence was placed on the results fro
m ITSZ. Intercompatibility tests were done for the representatives of
each taxon. In some species, intersterility had been found between rep
resentatives regarded as conspecific on morphological criteria. Such s
ibling species were not separated from each other in ITS2 sequences.