DETECTION OF PHIALOCONIDIA OF TRICHODERMA-HARZIANUM IN PEAT-BRAN BY MONOCLONAL ANTIBODY-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Murine monoclonal antibodies (MAbs) were raised against phialoconidia of a Trichoderma harzianum isolate T95 A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recogn ized, by enzyme-linked immunosorbent assay (ELISA), antigens from coni dia of T95. They did not react with antigens from mycelium or chlamydo spores of this isolate but recognized antigens from conidia of a numbe r of other T. harzianum and T. viride isolates. Heat, protease and per iodate treatment of T95 conidial antigens showed that GH5 MAbs recogni zed a protein epitope that formed part of a larger glycoprotein molecu le. GH5 MAbs were used to develop an ELISA for the detection of phialo conidia of T. harzianum in peat-bran.