A. Kinawi et al., FURTHER DEVELOPMENT OF FLOW-THROUGH ELECTROPHORESIS, European journal of clinical chemistry and clinical biochemistry, 34(1), 1996, pp. 49-52
Recent improvements in the field of flow-through-electrophoresis are p
resented. These improvements make it possible to run flow through elec
trophoresis with the same precision, but more economically and more ea
sily than the formerly described apparatus. This was achieved by limit
ing the original system to slab gels only. This leads to a considerabl
e reduction in the size of the apparatus and to simplification of its
handling. Although the gel width is reduced to 10 mm, the apparatus is
suitable for the analysis of mg as well as mu g quantities. The usefu
lness of this method is demonstrated by the analysis of samples obtain
ed during the isolation and purification of collagen type II, and by t
he analysis of insulin samples and other substances. The procedure is
also suitable for SDS-electrophoresis under flow-through conditions, e
nabling a simple and fast separation of proteins and determination of
their molecular masses.