SYNERGISTIC INHIBITORY EFFECT OF ENVIROXIME AND DISOXARIL ON POLIOVIRUS TYPE-1 REPLICATION

The effects of enviroxime, disoxaril and ribavirin in pair combination s on poliovirus type 1 (Mahoney) replication in FL cells were tested. Beforehand the fifty percent inhibitory concentration (IC50) was deter mined for each compound alone: enviroxime - 0.2 mu mol/l, disoxaril - 0.3 mu mol/l, ribavirin - 3 mu mol/l. Combining enviroxime with disoxa ril resulted in synergistic interaction, while combinations with ribav irin were markedly antagonistic. Enviroxime- and disoxanil-resistant p oliovirus mutants appeared following 10 and 2 consecutive passages in FL cells, respectively. No cross-resistance was observed between these mutants towards disoxaril and enviroxime, respectively.