IMPROVED SINGLE-STEP PCR ASSAY FOR SEX IDENTIFICATION POST-ALLOGENEICSEX-MISMATCHED BMT

Authors
PUGATSCH T OPPENHEIM A SLAVIN S
Citation
T. Pugatsch et al., IMPROVED SINGLE-STEP PCR ASSAY FOR SEX IDENTIFICATION POST-ALLOGENEICSEX-MISMATCHED BMT, Bone marrow transplantation, 17(2), 1996, pp. 273-275
Citations number
3
Categorie Soggetti
Hematology,Oncology,Immunology,Transplantation
Journal title
Bone marrow transplantation → ACNP
ISSN journal
02683369
Volume
17
Issue
2
Year of publication
1996
Pages
273 - 275
Database
ISI
SICI code
0268-3369(1996)17:2<273:ISPAFS>2.0.ZU;2-B
Abstract
A method is presented which allows sex-identification simultaneously o f both male and female cells in cell mixtures, The sensitivity of this PCR-based assay is one male cell in 10(6) female cells, The test can be used routinely to analyze bone marrow and peripheral blood lymphocy tes post-allogeneic sex-mismatched bone marrow transplantation to dete ct early engraftment or rejection, as well as host cell regeneration.