B. Escobar et al., A METHOD FOR EVALUATING THE PROPORTION OF FREE AND ATTACHED BACTERIA IN THE BIOLEACHING OF CHALCOPYRITE WITH THIOBACILLUS-FERROOXIDANS, Hydrometallurgy, 40(1-2), 1996, pp. 1-10
A method for evaluating the proportion of bacteria attached to mineral
sulfides is presented. The method is based on the determination of th
e rate of ferrous iron oxidation by attached bacteria on the mineral s
urfaces. The values obtained with this procedure are in agreement with
measurements of attached bacteria using bacteria grown with radioacti
ve (C-14) NaHCO3. The methodology was used to evaluate the fraction of
bacteria attached to chalcopyrite during bioleaching of this sulfide.
It was observed that a significant fraction of the bacteria grow atta
ched to the mineral.