STRUCTURES OF LIPOPOLYSACCHARIDE SIDE-CHAINS OF NCPPB-871, THE NEOPATHOTYPE STRAIN OF PSEUDOMONAS-SYRINGAE PV APTATA, STRAIN 299A OF PV PISI AND NCPPB-52, THE NEOPATHOTYPE STRAIN OF PV PHASEOLICOLA, IN RELATION TO O-SEROGROUP

The side-chains of lipopolysaccharides from Pseudomonas syringae pvs. aptata, pisi and phaseolicola contain D-rhamnose. Using H-1- and C-13- nuclear magnetic resonance spectroscopy and methylation analysis, the side-chains of NCPPB 871, the neopathotype strain of pv. aptata, and s train 299A of pv, pisi race 1 were found to be of identical structure: [GRAPHICS] Side-chains from NCPPB 52, the neopathotype strain of pv. phaseolicola, were based on the same backbone structure, but were subs tituted to the extent of approximately 67% with branch D-fucose residu es: [GRAPHICS] Side-chain structures are correlated with a previously proposed serogrouping system.