Phytoplasmas were constantly detected by the DAPI technique and by PCR
amplification of DNA From European plums (Prunus domestica) cv 'Susin
a di Dro' with symptoms similar to those reported for Japanese plum le
ptonecrosis. These symptoms included upward rolling of the leaves, whi
ch also became bronzed-reddish, thick and brittle, growth of normally
dormant axillary buds, off-season growth during November-January and p
hloem necrosis. Trees with symptoms showed progressive decline. RFLP a
nalysis revealed that the phytoplasma isolated from the affected plum
trees is closely related to the other phytoplasmas present in Prunus s
pecies in Europe and can be included in the apple proliferation cluste
r. Although Japanese plum leptonecrosis is common in Europe, this is t
he first report of a wide-spread phytoplasma-induced decline in a cult
ivar of European plum.