SUBSTRATE PROPERTIES OF ADENOSINE-3'-PHENYLPHOSPHONATES AND URIDINE-3'-PHENYLPHOSPHONATES FOR 3'-NUCLEOTIDASE NUCLEASES/

Authors
UCHIDA H WU YD SONODA S FUKUSHIMA S FUKUDA J TAKAHASHI I MAEDA S NOMURA A
Citation
H. Uchida et al., SUBSTRATE PROPERTIES OF ADENOSINE-3'-PHENYLPHOSPHONATES AND URIDINE-3'-PHENYLPHOSPHONATES FOR 3'-NUCLEOTIDASE NUCLEASES/, Nucleosides & nucleotides, 15(1-3), 1996, pp. 649-654
Citations number
23
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
15
Issue
1-3
Year of publication
1996
Pages
649 - 654
Database
ISI
SICI code
0732-8311(1996)15:1-3<649:SPOAAU>2.0.ZU;2-Q
Abstract
Adenosine- and uridine-3'-phenylphosphonates have been synthesized and evaluated as substrates of 3'-nucleotidase/nucleases. No other nuclea ses hydrolyzed these compounds. Since V-max values for the adenosine d erivative were comparable to those for 3'-AMP and the apparent K-m wer e 1.4-2.6 mM, it may be a useful substrate.