N-2-(P-N-OCTYLPHENYL)DGTP - SYNTHESIS AND INHIBITORY ACTIVITY AGAINSTDNA-POLYMERASES

Authors
JANSONS J STATTEL J VERRI A GAMBINO J KHAN N FOCHER F SPADARI S WRIGHT G
Citation
J. Jansons et al., N-2-(P-N-OCTYLPHENYL)DGTP - SYNTHESIS AND INHIBITORY ACTIVITY AGAINSTDNA-POLYMERASES, Nucleosides & nucleotides, 15(1-3), 1996, pp. 669-682
Citations number
20
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
15
Issue
1-3
Year of publication
1996
Pages
669 - 682
Database
ISI
SICI code
0732-8311(1996)15:1-3<669:N-SAIA>2.0.ZU;2-3
Abstract
N-2-(p-n-Octylphenyl)-2'-deoxyguanosilne 5'-triphosphate (OctPdGTP) ha s been synthesized chemically. OctPdGTP inhibited DNA polymerases (pol ) alpha, delta and epsilon from calf thymus, with moderate selectivity for pol alpha. Mechanistic studies on pol alpha and bacteriophage T4 DNA polymerase revealed competitive and mixed kinetics of OctPdGTP wit h respect to the substrate dGTP when the enzymes were assayed on activ ated DNA and oligo dT:poly dA, respectively.