CHEMICALLY DERIVED PEPTIDE LIBRARIES - A NEW RESIN AND METHODOLOGY FOR LEAD IDENTIFICATION

We have developed a new resin for peptide synthesis that can be used t o synthesize and evaluate directly combinatorial peptide libraries for binding target proteins. Fidelity of the peptide synthesis using this hydrophilic resin is comparable to polystyrene-based resins. Peptide libraries synthesized on this resin were probed by a two color PEptide Library Immunostaining Chromatographic ANalysis (PELICAN) technique f or sequences binding the serine protease Factor IX zymogen. This PELIC AN technique readily distinguishes between beads interacting with the reagents for target detection (blue beads) from those beads specific f or the target protein itself (red beads). Validation of the PELICAN te chnique, as well as purification of Factor IX from plasma, is demonstr ated utilizing this resin. (C) Munksgaard 1996.