Ja. Buettner et al., CHEMICALLY DERIVED PEPTIDE LIBRARIES - A NEW RESIN AND METHODOLOGY FOR LEAD IDENTIFICATION, International journal of peptide & protein research, 47(1-2), 1996, pp. 70-83
We have developed a new resin for peptide synthesis that can be used t
o synthesize and evaluate directly combinatorial peptide libraries for
binding target proteins. Fidelity of the peptide synthesis using this
hydrophilic resin is comparable to polystyrene-based resins. Peptide
libraries synthesized on this resin were probed by a two color PEptide
Library Immunostaining Chromatographic ANalysis (PELICAN) technique f
or sequences binding the serine protease Factor IX zymogen. This PELIC
AN technique readily distinguishes between beads interacting with the
reagents for target detection (blue beads) from those beads specific f
or the target protein itself (red beads). Validation of the PELICAN te
chnique, as well as purification of Factor IX from plasma, is demonstr
ated utilizing this resin. (C) Munksgaard 1996.