Ar. Mcquiston et al., NEUROPEPTIDE Y-1 RECEPTORS INHIBIT N-TYPE CALCIUM CURRENTS AND REDUCETRANSIENT CALCIUM INCREASES IN RAT DENTATE GRANULE CELLS, The Journal of neuroscience, 16(4), 1996, pp. 1422-1429
Neuropeptide Y (NPY) is far more abundant in the dentate gyrus than el
sewhere in the hippocampal formation, but it does not alter the synapt
ic excitation of dentate granule cells (DGCs) as it does for pyramidal
cells in areas CA1 and CA3. NPY inhibited depolarization-induced incr
eases in intracellular Ca2+ concentrations ([Ca2+](i)) in DGCs in hipp
ocampal slices, without altering the resting [Ca2+](i). NPY inhibited
Ca2+ currents (I-Ca) via a Y-1 receptor in 84% of acutely isolated DGC
s and via a Y-2 receptor in 31% of the NPY-responsive cells tested. I-
Ca inhibition was completely occluded by omega-conotoxin-GVIA but not
by nimodipine. The inhibition of I-Ca was accompanied by a change in t
he lime course of I-Ca activation in only 27% of NPY-responsive cells.
Only 23% of DGCs responded to NPY when Ba2+ was substituted for extra
cellular Ca2+ and when [Ca2+](i) was strongly buffered. Therefore, NPY
inhibits an N-type I-Ca in DGCs, mainly via Y-1 receptors. Furthermor
e, it seems that more than one mechanism, one of which may be sensitiv
e to [Ca2+](i), may couple NPY receptors to the Ca2+ channels in DGCs.
Because the release of dynorphin from DGCs depends in part on N-type
currents, NPY receptors are poised to regulate the release of opioid p
eptides from DGC somata and dendrites.