CLONING, SEQUENCING AND EXPRESSION OF THE AMYLASE ISOZYME GENE FROM PSEUDOMONAS SP KFCC-10818

Authors
KIM ES NA HK JHON DY YOO OJ CHUN SB WUI IS
Citation
Es. Kim et al., CLONING, SEQUENCING AND EXPRESSION OF THE AMYLASE ISOZYME GENE FROM PSEUDOMONAS SP KFCC-10818, Biotechnology letters, 18(2), 1996, pp. 169-174
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
2
Year of publication
1996
Pages
169 - 174
Database
ISI
SICI code
0141-5492(1996)18:2<169:CSAEOT>2.0.ZU;2-M
Abstract
A gene coding for amylase was cloned and sequenced from an alkalophili c Pseudomonas sp. KFCC 10818. The coding region for the amylase precur sor contained 1,692 nucleotides. The presumed Shine-Dalgarno sequence, AAGG, was located at 8 nucleotides upstream from the ATG initiation c odon. The precursor protein had a putative signal peptide of 25 amino acid residues at its amino terminus. The Pseudomonas amylase had four highly conserved regions as other cr-amylases. The amylase expressed f rom E. coli harboring the Pseudomonas gene produced maltose and maltot riose from soluble starch.