Sm. Fitzjohn et al., ACTIVATION OF GROUP-I MGLURS POTENTIATES NMDA RESPONSES IN RAT HIPPOCAMPAL SLICES, Neuroscience letters, 203(3), 1996, pp. 211-213
The pharmacology of the metabotropic glutamate receptor (mGluR)-mediat
ed potentiation of N-methyl-D-aspartate (NMDA)-evoked depolarisations
in the CA1 region of rat hippocampal slices was investigated using an
extracellular grease-gap method. The group I and II mGluR agonist (1S,
3R)-1-aminocyclopentane-1,3-dicarboxylic acid ((1S,3R)-ACPD; 10 mu M)
potentiated responses to NMDA (15-25 mu M), giving a dose ratio of 0.8
4 +/- 0.02. The mGluR group I specific agonist (RS3,5-dihydroxyphenylg
lycine (DHPG) (3-10 mu M) also induced a dose-dependent and reversible
enhancement of responses to NMDA (dose ratio for 10 mu M DHPG was 0.7
7 +/- 0.02). In contrast, the group II selective agonist 1'R,2'R,3'R)-
2-(2',3'-dicarboxycyclopropyl)glycine (DCG-IV; 0.5-1 mu M) and the gro
up III specific agonist (S)-2-amino-4-phosphonobutanoate (L-AP4; 50 mu
M) caused little or no potentiation of responses to NMDA. The potenti
ation induced by 3-5 mu M DHPG was reversibly antagonised by the group
I and II antagonist (+)-alpha-methyl-4-carboxyphenylglycine ((+)-MCPG
; 1 mM). The present findings demonstrate that activation of group I m
GluRs enhance NMDA responses in the hippocampus.