ASSIGNMENT OF THE HUMAN FAST SKELETAL TROPONIN T GENE (TNNT3) TO CHROMOSOME 11P15.5 - EVIDENCE FOR THE PRESENCE OF 11PTER IN A MONOCHROMOSOME-9 SOMATIC-CELL HYBRID IN NIGMS MAPPING PANEL-2

Human fast skeletal troponin T (TnT(f)), the tropomyosin binding compo nent of the multisubunit troponin complex, plays an important role in the Ca2+ regulation of striated muscle contraction. Specific primers d esigned from the 3' end of human TnT(f) cDNA were used to amplify an i ntronic region by polymerase chain reaction (PCR). This TnT(f)-specifi c PCR product was detected from two somatic cell hybrids containing hu man chromosomes 9 and 11, respectively, in MG;MS mapping panel 2, Howe ver, further studies with other somatic hybrid cell lines (Bios Labora tory) localized the TnT(f) gene (HGMW-approved symbol TNNT3) only to c hromosome 11, This observation was further confirmed by fluorescence i n situ hybridization with a 12-kb TnT(f) genomic probe generated by ex tended PCR, showing the sublocalization of the gene to band p15.5 on c hromosome 11. This locus is of specific interest, as Beckwith-Wiedeman n syndrome and various childhood and adult tumor-related abnormalities have been mapped to this region. The study also indicates the presenc e of an 11pter region in the NIGMS cell hybrid GM10611, which has prev iously been reported to contain only human chromosome 9. (C) 1996 Acad emic Press, Inc.