A. Fehrmann et al., DAIRY PRODUCT ANALYSIS - IDENTIFICATION OF MICROORGANISMS BY MIDINFRARED SPECTROSCOPY AND DETERMINATION OF CONSTITUENTS BY RAMAN-SPECTROSCOPY, Journal of AOAC International, 78(6), 1995, pp. 1537-1542
Identification of microorganisms by traditional microbiological method
s is time consuming. The German Federal Health Office has developed a
method using mid-infrared spectroscopy to identify microorganisms rapi
dly. This method has been modified for application to microorganisms i
mportant in the dairy industry. Mid- and near-infrared spectroscopies
are well-established methods for quantitative measurements of fat, pro
tein, lactose, and solid content in a variety of products. A disadvant
age of both methods is the huge absorption due to water; extraction of
other components is complicated and can be achieved only statisticall
y. With Raman spectroscopy, water causes less absorption. We investiga
ted the use of Raman spectroscopy as a quantitative method for milk po
wder.