IDENTIFICATION OF OSTEOCALCIN AS A PERMANENT AGING CONSTITUENT OF THEBONE-MATRIX - BASIS FOR AN ACCURATE AGE AT DEATH DETERMINATION

Age at death determination based on aspartic acid racemization in dent in has been applied successfully in forensic odontology for several ye ars now. An age-dependent accumulation of D-aspartic acid has also rec ently been demonstrated in bone osteocalcin, one of the most abundant noncollagenous proteins of the organic bone matrix. Evaluation of thes e initial data on in vivo racemization of aspartic acid in bone osteoc alcin was taken a step further. After purification of osteocalcin from 53 skull bone specimens, the extent of aspartic acid racemization in this peptide was determined. The D-aspartic acid content of purified b one osteocalcin exhibited a very close relationship to age at death. T his confirmed identification of bone osteocalcin as a permanent, 'agin g' peptide of the organic bone matrix. Its D-aspartic acid content may be used as a measure of its age and hence that of the entire organism . This new biochemical approach to determination of age at death by an alyzing bone is complex and demanding from a methodologic point of vie w, but appears to be superior in precision and reproducibility to most other methods applicable to bone.