Kn. Allen et al., AN EXPERIMENTAL APPROACH TO MAPPING THE BINDING SURFACES OF CRYSTALLINE PROTEINS, Journal of physical chemistry, 100(7), 1996, pp. 2605-2611
Porcine pancreatic elastase has been used as the model enzyme in the d
esign and development of a crystallographic method that allows mapping
of the binding surface of a protein by solving its crystal structure
in a variety of organic solvents. The ultimate goal of this method is
to aid in the process of drug design, where each of the chosen organic
molecules represents a given functional group in a larger inhibitor m
olecule. This method of multiple solvent crystal structures (MSCS) has
a theoretical counterpart in the method of multiply copy simultaneous
search (MCSS) (Miranker, A.; Karplus, M. Proteins: Struct., Funct., G
enet. 1991, 11, 29-34) and is the first experimental method that can b
e used as a check to the theory. The MSCS method is presented here wit
h acetonitrile as the probe organic solvent. The procedure involved do
es not cause significant changes in the structure of elastase as compa
red to the structure in aqueous solution, and the positions found for
the acetonitrile molecules in the active site are compared to those of
similar functional groups belonging to known inhibitors bound to elas
tase.