ESTABLISHMENT AND CHARACTERIZATION OF 2 CELL-LINES DERIVED FROM A PRIMARY ADENOCARCINOMA OF THE DUODENUM

Aims-To establish two cell. lines from a primary duodenal adenocarcino ma; to describe the morphological, growth, ploidy, and immunophenotypi c characteristics of these cell lines. Methods-The cell lines, designa ted DAC/S and DAC/E, were characterised using both in vitro and in viv o cell culture techniques, light and electron microscopy, immunocytoch emistry, and FAGS analyses. Results-Both cell lines have an epithelial origin, are aneuploid and display characteristics of transformed cell s. The cell lines differ from each other in morphology, doubling time and serum requirements. These cell lines are anchorage dependent and d o not grow in nude mice. Conclusions-DAC/S and DAC/E cell lines are de rived from neoplastic epithelium and could provide in vitro model syst ems for future investigations of the cell and molecular biology of duo denal neoplasia.