SIMULTANEOUS MEASUREMENTS OF GLUCOSE, GLUTAMINE, AMMONIA, LACTATE, AND GLUTAMATE IN AQUEOUS-SOLUTIONS BY NEAR-INFRARED SPECTROSCOPY

Calibration models are generated and evaluated for the measurement of five different components in synthetic mixtures prepared in aqueous so lutions. Mixtures of glucose, glutamine, ammonia, lactate, and glutama te were prepared to simulate concentration levels expected during rout ine bioreactor fermentation processes. Near-IR spectra were collected from these solutions over the spectral range from 5000 to 4000 cm(-1). This spectral information was used to build individual multivariate c alibration models for each analyte. Models were constructed on the bas is of partial least-squares regression of raw and Fourier filtered abs orbance spectra. Each analyte could be detected selectively with mean percent errors of prediction ranging from 4 to 8%.