BIOCATALYTIC PRODUCTION OF USEFUL ESTERS BY 2 FORMS OF LIPASE FROM CANDIDA-RUGOSA

Two distinct lipase forms were obtained from Candida rugosa lipase by Phenyl Sepharose hydrophobic interaction and DEAE Sepharose ion exchan ge chromatography, L1 at 45% yield and L2 at 4.7% yield. Both purified lipases were able to catalyse esterification of 1-butanol and oleic a cid and transesterification of 2-ethyl-1-hexanol and rapeseed oil. Lip ase L1 gave a 98% yield for esterification over 12 h and a 99% convers ion of rapeseed oil for transesterification over 24 h. The minor fract ion L2 gave a 97% yield for esterification over 30 h and only a 79% co nversion for trans-esterification over 24 h. The superiority of fracti on L1, especially in trans-esterification, could be clearly shown by r eversed phased HPLC analysis. Sodium deoxycholate treatment of the pur ified main lipase L1 considerably improved the initial rate in both es terification and trans-esterification.