BIOCHEMICAL-CHARACTERIZATION OF THE RECOMBINANT BOOPHILUS-MICROPLUS BM86 ANTIGEN EXPRESSED BY TRANSFORMED PICHIA-PASTORIS CELLS

Authors
MONTESINO R CREMATA J RODRIGUEZ M BESADA V FALCON V DELAFUENTE J
Citation
R. Montesino et al., BIOCHEMICAL-CHARACTERIZATION OF THE RECOMBINANT BOOPHILUS-MICROPLUS BM86 ANTIGEN EXPRESSED BY TRANSFORMED PICHIA-PASTORIS CELLS, Biotechnology and applied biochemistry, 23, 1996, pp. 23-28
Citations number
29
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biotechnology and applied biochemistry → ACNP
ISSN journal
08854513
Volume
23
Year of publication
1996
Part
1
Pages
23 - 28
Database
ISI
SICI code
0885-4513(1996)23:<23:BOTRBB>2.0.ZU;2-G
Abstract
In the present paper we report the biochemical characteristics of the recombinant tick (Boophilus microplus) gut antigen Bm86 that previousl y has been cloned, expressed and recovered at high levels in the methy lotrophic yeast Pichia pastoris. The results demonstrate that rBm86 ha d a modification at position 92 (Thr replaced by Ile) and aggregated, forming particles ranging between 17 and 40 nm. The rBm86 was N-glycos ylated, having at least two non-glycosylated sequons (Asn-329 and Asn- 363) and a ratio of only 0.4/65 (free Cys/total Cys)/mol of protein.