M. Costaferreira et al., SYNTHETIC LIGNIN DEGRADATION AND PEROXIDASE FORMATION BY PHANEROCHAETE MAGNOLIAE, Biotechnology and applied biochemistry, 23, 1996, pp. 37-40
Phanerochaete magnoliae mineralized synthetic lignin in low-nitrogen m
edia provided that the Mn(II) concentration was kept low (about 0.5 p.
p.m.). The amount of [C-14]dehydrogenative polymerizate (synthetic lig
nin) ([C-14]DHP) oxidized to (CO2)-C-14 was about 10-15% of the total
added. Two simple cultivation parameters, the concentration of Mn(II)
and temperature, were used as tools to obtain widely different titres
of extracellular peroxidases, which were examined in relation to [C-14
]DHP mineralization. Both lignin peroxidase and Mn peroxidase accumula
ted in the extracellular broth of P. magnoliae unusually early but [C-
14]DHP mineralization was only observed about 3 days alter the onset o
f peroxidase production. Aspects of [C-14]DHP mineralization and perox
idase formation by P. magnoliae were compared with those reported for
P. chrysosporium.