SPECTROSCOPIC AND THERMODYNAMIC CHARACTERIZATION OF THE ADSORPTION OFPLASMA-PROTEINS ONTO CELLULOSIC SUBSTRATES

The sorption of the plasma proteins human serum albumin (HSA) and huma n fibrinogen (FIB) onto hemodialytic cellulosic substrates was investi gated by the surface sensitive ATR-FTIR-spectroscopy. By means of this method we monitored the protein sorption kinetics onto acetylated and unmodified cellulose (AKZO-NOBEL). Furthermore, secondary structure a lterations of the adsorbed proteins as well as changes of the composit ion in sorbed layers consisting of two proteins were detected. These f indings were compared with results of the zeta potential and contact a ngle measurements on comparable sorption experiments.