Hybrid seed production can be facilitated by using male sterility coup
led with a seedling marker, This research was initiated to combine the
ms male sterility and dg delayed-green seedling marker into watermelo
n [Citrullus lanatus (Thunb,) Matsum. & Nakai] lines, Male-sterile pla
nts of the male-sterile line G17AB were crossed with plants of delayed
-green breeding line Pale90, which has yellow cotyledons and pale-gree
n, newly developed, true leaves, The double-recessive recombinants, ma
le sterile and delayed green, from the F-2 population were backcrossed
to the male-fertile plants of G17AB, The pedigree method was used for
selection in the progenies, The segregation ratios obtained from F-2
and BC1F2 populations suggest that the male-sterile and delayed-green
traits are inherited independently and that delayed green is inherited
as a single recessive nuclear gene, Two male-sterile watermelon lines
with delayed-green seedling marker have been developed, These lines w
ill provide a convenient way to introduce male sterility and the delay
ed-green seedling marker into various genetic backgrounds, These two l
ines can be used for testing the efficiency of a new, hybrid, watermel
on, seed production system.