Pepper (Capsicum annuum L. 'Early Calwonder') leaf disks were vacuum-i
nfiltrated in distilled water (control), anisomycin, aurintricarboxyli
c acid, cycloheximide, ethionine, norvanine, or puromycin to determine
whether protein synthesis inhibitors blocked high-temperature acclima
tion. After infiltration, one-half of the leaf disks were placed in an
incubator at 24C as a control, and the other half were kept in a wate
r bath at 38C for 2 h to induce acclimation. Test tubes containing the
disks then were placed in a water bath at 50.5C for 0, 1, 5, 10, 15,
25, 35, or 50 minutes. Thermotolerance was evaluated using electrolyte
leakage. High-temperature acclimation was blocked in all six protein
synthesis-inhibitor treatments. Only control disks infiltrated with di
stilled water acclimated. It seems that protein synthesis is required
for high-temperature acclimation in bell pepper leaves.