Campanula medium L. hairy roots induced by infection with Agrobacteriu
m rhizogenes A(13) grew well in three hormone-free liquid media, Muras
hige-Skoog (MS), Gamborg B5 (B5) and Woody Plant (WP). In these media,
the hairy roots produced a high amount of the polyacetylene monogluco
side, lobetyolin (maximum content: 3.74% in MS medium, 2.69% in B5 med
ium and 3.13% in WP medium, dry weight) whose levels were over 130 tim
es higher than that of the intact plant.