4-Picoline-2,2':6',2''-terpyridine-platinum(II) is shown in a ligation
assay to unwind and so intercalate into DNA, Circular dichroism is us
ed to determine an equilibrium binding constant of approximately 2 x 1
0(7) M(-1) for the most stable binding mode of 3-picoline-2,2': 6',2''
-terpyridine-platinum(II) to poly[d(A-T)(2)] with a site size of about
4 base pairs, and about 1 x 10(6) M(-1) for a second binding mode wit
h a site size of about 2 base pairs. Fluorescence spectroscopy provide
s further evidence for the strong equilibrium binding constant of 4-pi
coline-2,2': 6',2''-terpyridine-platinum(II) in that it displaces ethi
dium bromide bound to DNA, The double positive charge on 4-picoline-2,
2': 6',2''-terpyridine-platinum(II) , together with the intercalative
binding mode is probably responsible for the large binding constant.