DOUBLE LONG-CHAIN AMIDINE LIPOSOME-MEDIATED SELF-REPLICATING RNA TRANSFECTION

Authors
ELOUAHABI A PECTOR V FUKS R VANDENBRANDEN M RUYSSCHAERT JM
Citation
A. Elouahabi et al., DOUBLE LONG-CHAIN AMIDINE LIPOSOME-MEDIATED SELF-REPLICATING RNA TRANSFECTION, FEBS letters, 380(1-2), 1996, pp. 108-112
Citations number
23
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
380
Issue
1-2
Year of publication
1996
Pages
108 - 112
Database
ISI
SICI code
0014-5793(1996)380:1-2<108:DLALSR>2.0.ZU;2-A
Abstract
We present experimental evidence that a complex made of a double long chain cationic amphiphile and recombinant mRNA facilitates the entry a nd expression of genetic material into cells. Combining the properties of the self replicating recombinant mRNA driven by the Semliki Forest Virus (SFV) replicon and the transfection potentialities of a new cat ionic amphiphile tyl-N'-tetradecyl-3-tetradecylaminopropionamidine) yi elds a highly efficient mRNA transfection system conferring up to 100% infectivity. The preparation and characterization of the long chain a midine cationic amphiphile-mRNA complex as well as the influence of th e diC14-amidine/RNA ratio on the infective activity are described.