Tg. Bernhardt et al., PURIFICATION OF FATTY-ACID ETHYL-ESTERS BY SOLID-PHASE EXTRACTION ANDHIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 675(2), 1996, pp. 189-196
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
We have developed a two-step method to purify fatty acid ethyl eaters
(FAEE) using solid-phase extraction (SPE), with a recovery of 70+/-3%
(mean+/-S.E.M.) as assessed using ethyl oleate as a recovery marker fr
om a standard lipid mixture in hexane. The first step of the SPE proce
dure involves application of a lipid mixture to an aminopropyl-silica
column with simultaneous elution of FAEE and cholesteryl esters from t
he column with hexane. Gas chromatographic analysis of FAEE without in
terference from cholesteryl esters may be performed using the eluate f
rom the aminopropyl-silica column, thus eliminating the need for an oc
tadecylsilyl (ODS) column in this case. The FAEE can then be separated
from the cholesteryl esters, if necessary, by chromatography on an OD
S column and elution with isopropanol-water (5:1, v/v). Both the amino
propyl-silica and ODS columns were found to be effective for up to fou
r uses. To permit isolation of specific FAEE species following isolati
on of total FAEE by the two-step SPE method, we have also developed a
purification scheme for individual FAEE by high-performance liquid chr
omatography (HPLC). Thus, this simple method allows for reproducible i
solation of total FAEE by SPE and isolation of individual FAEE species
by HPLC.