SIMPLE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY QUANTITATION OF GANCICLOVIR IN HUMAN SERUM AND URINE

A fast, simple, and cost-effective HPLC method for the quantitation of the antiviral drug ganciclovir is described. The serum samples are ex tracted with perchloric acid and neutralized with potassium phosphate buffer, and urine samples are diluted with distilled water. A reversed -phase column with isocratic elution by 15 mM potassium phosphate buff er (pH 2.5) containing 0.25% acetonitrile is used to separate ganciclo vir; quantitation is by UV absorbance at 254 nm. Total turnaround time is 22 min; more than 3000 samples can be run on a single column witho ut loss of peak quality. The limit of quantitation is 0.05 mu g/ml. Re coveries varied from 91 to 107% with coefficients of variation ranging from 0.387 to 7.95%.