B. Driessen et J. Goncalves, INHIBITION OF NEURAL ATP RELEASE BY ATRIAL-NATRIURETIC-PEPTIDE IN GUINEA-PIG VAS-DEFERENS, European journal of pharmacology, 296(3), 1996, pp. 261-265
Effects of atrial natriuretic peptide (ANP) and 8-bromoguanosine 3':5'
-cyclic monophosphate (8-BrcGMP) on contraction, overflow of tritium (
after [H-3]noradrenaline labelling) and overflow of ATP elicited by el
ectrical field stimulation (210 pulses/7 Hz) were studied in guinea-pi
g vas deferens. ANP (1-100 nM) slightly increased contractions, did no
t alter the overflow of tritium but decreased the overflow of ATP by u
p to 50%. 8-BrcGMP (3-300 mu M) markedly reduced contractions and ATP
overflow with no effect on tritium overflow. Contractions were suppres
sed in the presence of prazosin plus suramin, and evoked overflow of A
TP declined to 11%. ANP now gradually increased tritium overflow but a
gain decreased the overflow of ATP. 8-BrcGMP did not change tritium ov
erflow, as before, but increased ATP overflow. The results indicate th
at ANP inhibits neural release of ATP by a mechanism independent of gu
anylyl cyclase activation with no major effect on noradrenaline releas
e.