Ma. Subroto et al., DEVELOPMENT OF SHOOTY TERATOMAS FROM SEVERAL SOLANACEOUS PLANTS - GROWTH-KINETICS, STOICHIOMETRY AND ALKALOID PRODUCTION, Journal of biotechnology, 45(1), 1996, pp. 45-57
Shooty teratomas of Atropa belladonna and Solanum aviculare and shooty
teratomas and crown galls of a Duboisia leichhardtii X D. myoporoides
hybrid were established by infecting plants with nopaline and genetic
ally modified strains of Agrobacterium tumefaciens. Transformation was
confirmed by nopaline assay and Southern blotting. Patterns of growth
, substrate uptake and alkaloid production were studied in shake flask
s and bioreactors. Maximum specific growth rates of 0.25 d(-1) and 0.2
8 d(-1) were obtained for A. belladonna shooty teratomas and Duboisia
crown galls, respectively; specific growth rate declined throughout mo
st of the batch culture period. The elemental composition of A. bellad
onna shooty teratomas grown in MS medium was similar to that of other
plant cells. Growth of A. belladonna shoots in MS medium could be desc
ribed using a stoichiometric equation with constant yield coefficients
for biomass production from sugar, nitrate and ammonia. Alkaloid prod
uction in the shoot and gall cultures was limited, development of root
s significantly improved hyoscyamine synthesis by A. belladonna shooty
teratomas.